Western blot analysis of RAB33A in extracts of various cell lines. Samples were incubated with RAB33A Polyclonal antibody (Product # PA5-120525) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot analysis of RAB33A in various cell lines using a RAB33A Polyclonal antibody (Product # PA5-120525) at a dilution of 1:3,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supplier Page from Thermo Fisher Scientific for RAB33A Antibody